Omega-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are important for human health across the lifespan (1). While not essential nutrients, consumption of pre-formed EPA and DHA is recommended as conversion from their essential precursor, alpha-linolenic acid, is limited. In Australia, the suggested dietary target (SDT) for n-3 LCPUFA is 610 mg/day for males and 430 mg/day for females (2). This study estimated dietary n-3 LCPUFA intakes among Australian adults aged ≥18 years, excluding pregnant and breastfeeding women, using two 24-h dietary recalls from the 2023 National Nutrition and Physical Activity Survey (NNPAS; n = 4,101 males and n = 4,522 females) and compared intakes with those from the 2011-12 NNPAS (n = 4,328 males and n = 4,994 females). Usual n-3 LCPUFA intakes were estimated from both recall days using the multiple source method. Analyses were stratified by sex and age (18-44, 45-64, ≥ 65 years). Differences in median and mean intakes between survey years were assessed using a Wilcoxon rank-sum test and survey-adjusted F-tests with Bonferroni correction, respectively. Differences in the proportion achieving the SDT were assessed using survey-adjusted Pearson chi-square tests. Median (25th, 75th percentile) n-3 LCPUFA intakes were significantly lower in 2023 than in 2011-12 for both males (183 [135, 264] mg/day vs 197 [145, 281] mg/day, p < 0.001) and females (154 [118, 238] mg/day vs 170 [125, 255] mg/day, p < 0.001). Age-stratified analyses showed differences between survey years were significant in males 18-44 years (p < 0.001) and females 45-64 years (p < 0.01). The proportion achieving the SDT from food sources was low and, among males, significantly lower in 2023 than in 2011-12 (weighted % males: 3% vs 5%, p < 0.01; females: 7% vs 8%, p = 0.13). As in 2011-12, fish and seafood provided the largest amount of n-3 LCPUFA per gram of food in 2023 (8 mg/g), followed by eggs (1.5 mg/g), red meat and poultry (0.5 mg/g), cereals and grains (0.3 mg/g), and dairy (0.1 mg/g). N-3 LCPUFA supplement use, estimated from the first recall only, was lower in 2023 than in 2011-12 among males (9% vs 13%) and females (10% vs 17%; all p<0.001). Supplement users were more likely than non-users to meet the SDT in both survey years (males: 68% vs 9% in 2011-12; 56% vs 7% in 2023; females: 74% vs 11% in 2011-12; 63% vs 11% in 2023; all p<0.001). Dietary n-3 LCPUFA intakes among Australian adults have declined since 2011-12, despite already being low. Males aged 18-44 years and females aged 45-64 years appear particularly vulnerable to low intake. Given the very low proportion of adults meeting the SDT, strategies to improve n-3 LCPUFA intake are warranted.