South Asian-born mothers represent a growing demographic in Australia [1]. Optimal feeding during the first 2000 days of life is critical to mitigate this cohort’s increased risk for central adiposity, cardiovascular disease, and type 2 diabetes mellitus [2]. Despite this, there is limited evidence on the infant feeding practices of South Asian-born mothers in Australia or the socio-cultural barriers and facilitators that influence these practices. This limits opportunities to develop culturally appropriate interventions for optimal infant feeding practices and long-term health. Therefore, the aim of this study was to explore the knowledge, attitudes, behaviours, cultural beliefs, intentions and support influencing infant feeding practices among South Asian-born mothers living in Australia. This was a qualitative study consisting of semi-structured telephone interviews, with a sample of South Asian-born mothers living in Australia with children aged 1.5-5.0 years. Recruitment was conducted nationwide via a Facebook advertisement campaign and targeting relevant cultural groups and organisations. Data were analysed using inductive thematic analysis guided by an interpretivist epistemological paradigm. Seventeen South Asian-born mothers completed the interview, with countries of birth including Pakistan (n = 9), Sri Lanka (n = 4), Bangladesh (n = 3), and Afghanistan (n = 1). The age ranged from 26 years to 37 years. All the mothers were married and living with their spouse and children; of these, two reported that their in-laws also resided in the home. Languages spoken at home included Bangla, Dari, Farsi, Punjabi, Sindhi, Sinhalese, Tamil, Turkish and Urdu. The number of years living in Australia ranged from 3.5 years to 28 years. All mothers reported having completed a tertiary qualification (Bachelor, Masters or PhD). Eight mothers reported having gestational diabetes during any of their pregnancies. Four themes emerged relating to infant feeding practices among South Asian-born mothers: 1) Physical and emotional challenges highlighted the barriers to feeding including exhaustion, lactation difficulties and recovery from giving birth (caesarean sections), 2) Missing family support and isolation, the absence of family and the resulting challenges of managing infant care independently, 3) Navigating culture and medical advice, captured how mothers balanced advice from healthcare professionals and their cultural traditions, and 4) Trusting intuition over guidelines, showed how mothers adjusted their feeding practices, relying on peer advice and adopting early mixed feeding methods and introduction of solids. To effectively assist South Asian-born mothers in infant feeding practices, the health professionals should offer tailored clinical support that facilitates maternal postpartum recovery. Integrating cultural and religious practices into standard care may aid in encouraging, motivating and supporting South Asian-born mothers in their infant feeding journey.